In vitro neuroprotection screen against human-tau-oligomerinduced neurotoxicity in rodent neurons
Rodent primary cortical neurons (6 days in vitro) are challenged with e.g. 1 µM SynAging's hTO preparation for 24 hours. The hTO concentration given are based on used tau monomer. Client’s therapeutic candidates are usually applied together with this challenge.The following read-outs are established at SynAging: MTT, Calcein / PI, LDH release, ATP lite, and ELISA of NSE (neuro specific enolase). Humanin and trolox (shown below) have been established as positive control.
Figure right: In a comparison of tau monomer (Tau-M), tau oligomer (Tau-O), tau fibrils (Tau-F), and sonicated tau fibrils (Tau-F Sonicated), the highest toxicity of oligomers is obvious.
hTO have also been established to induce inflammatory cytokine release from primary rodent astrocytes:
Mouse primary astrocytes (DIV 7) were treated with 2 or 5 µM hTO. Readout is ELISA of IL1β, TNFα, and IL6 after 1 hour.
SynAging is evaluating client therapies for their potential to interfere with hTO-induced inflammatory cytokine release in this in vitro model.